Police have charged three men and interviewed a teenager after four kangaroos were injured at Jack Smith Lake Game Reserve on Thursday night.

The men were arrested on Friday morning at about 11am after they were found with a stolen tractor in the reserve on Middle Rd.

A 21-year-old man from Athlone has been charged with theft of a motor vehicle, criminal damage, possessing a firearm while unlicensed and cruelty to animals offences.

He has been bailed to appear at the Latrobe Valley Magistrates Court on September 15.

A 20-year-old Drouin South man is expected to be charged on summons for permitting an unlicensed person to posses a firearm, hunting in a game reserve out of season and hunting protected animals.

An 18-year-old Warrigal man is expected to be charged on summons for cruelty to animals.

The Department of Sustainability and Environment is assisting police with their investigations.

Police allege the men were running over kangaroos in their ute at the reserve between 9pm and 11pm on Thursday.

It is also alleged the men were shooting at mobs of kangaroos but police are unsure if any were injured.

Police said the ute the men were travelling in became bogged in the mud while they were out on the reserve and they allegedly stole a tractor from a nearby property.

Further charges are pending for other wildlife offences.

The Darriman kangaroo attacks follow on from an incident in Loch Sport almost two months ago where hoons killed two kangaroos while ripping up the fairways of the Loch Sport Golf Course.

Meanwhile police and DSE are worried about the welfare of a deer seen wandering around Loch Sport in recent weeks with an arrow sticking out of its neck.

Police are investigating a possible case of illegal hunting of deer using a bow and arrow close to the town.

The deer has been sighted several times and police and DSE wildlife officers are trying to find the hog deer to determine if it can be treated or if it needs to be put down.

It is believed the deer has had the arrow stuck in its neck for more than two weeks.

The last sighting of the deer was on Thursday afternoon on Loch Sport Rd 500m out of town.

Anyone who has seen the deer is asked to contact Sale police on 51435000.